MINI PARASEP®
STEP 1
Sample Preparation: For empty Parasep®, unscrew lid and add 2.4ml of fixative and one drop of surfactant (eg: Apacor Triton X solution) to the mixing chamber. Alternatively use the reagent ready Mini Parasep®. Introduce a pea sized faecal sample to the fixative using the spoon on the end of the Mini Parasep® filter. Add 1.0ml of Ethyl Acetate to the mixing chamber. Mix in thoroughly with the Mini Parasep® spoon. If the sample is hard, break it up with the end of the spoon.
STEP 2
Emulsification: Seal the Mini Parasep® by screwing in the filter/ sedimentation cone unit. Vortex or shake to emulsify with the sedimentation cone pointing upwards.
STEP 3
Centrifugation: Invert the Mini Parasep® and centrifuge at 1200g for three minutes. Mini Parasep® fits all 15ml centrifuge buckets
STEP 4
Examination: Unscrew and discard the filter and mixing tube. Pour off all the liquid above the sediment. Pipette one drop of sediment onto a slide and cover with coverslip. Alternatively, follow laboratory SOP for slide preparation.
