STEP 1:

Sample Preparation

A) Unscrew lid.
B) Add 2.4ml of fixative.
C) Add one drop of surfactant (eg: Apacor Triton X solution)
to the chamber.
Alternatively, use reagent ready Mini Parasep®.
D) Introduce a level scoop of formed faecal sample(equivalent 0.5g) to the fixative using the spoon at the end of the Mini Parasep® filter. For liquid samples, add 2 level scoops (equivalent 1g) to the fixative. Add 1.0ml of Ethyl Acetate to the mixing chamber. Mix in thoroughly with the Mini Parasep® spoon. If the sample is hard, break it up with the end of the spoon.

STEP 2:

Emulsification

A) Seal Mini Parasep® by screwing in the filter/sedimentation cone unit. Vortex or shake to emulsify with the sedimentation cone pointing upwards.

STEP 3:

Centrifugation

A) Invert Mini Parasep® and centrifuge at 1200g for 3 minutes. Mini Parasep® fits all 15ml centrifuge buckets.