MINI PARASEP® SF US PROTOCOL
Step 1: PREPARATION
Preserved Samples: Shake or vortex the incoming preserved sample to thoroughly mix. Transfer either 2ml (US Gold Standard) or 3ml (ARUP JCM) of the emulsified stool into the Mini Parasep® SF mixing chamber. In the event of: Thick Stool Samples—please add 10 drops of Apacor Triton X solution, then please enclose and vortex/shake to emulsify prior to transferring the sample; Liquid Stool Samples—please add 4ml instead of 2ml or 3ml to ensure that a sediment is formed after centrifugation is performed.
Step 2: EMULSIFICATION
Seal the Mini Parasep® SF by screwing in the filter/ sedimentation cone unit.
Step 3: CENTRIFUGATION
Invert Mini Parasep® SF and perform centrifugation at: 500g for ten minutes (US Gold Standard); 400g for two minutes (ARUP JCM).
Step 4: EXAMINATION
Unscrew and discard the filter and mixing tube. Decant the supernatant. Transfer sediment to slide to perform examination where all temporary (Lugol's Iodine) and permanent staining techniques (Trichrome) can be conducted providing a universal fixative is used. If a universal fixative is used in conjunction with this device, then molecular and EIA tests can be conducted from the sediment.
