MINI PARASEP® SF EU PROTOCOL
Step 1: PREPARATION
Fresh Samples For empty Parasep®, unscrew lid and add 3.3ml of fixative and one drop of surfactant (eg: Apacor Triton X solution) to the mixing chamber. Alternatively use the reagent ready Mini Parasep® SF. Introduce a level scoop of faecal sample to the fixative using the spoon on the end of the Mini Parasep® SF filter. Mix in thoroughly.
Step 2: EMULSIFICATION
Seal the Mini Parasep® SF by screwing in the filter/ sedimentation cone unit. Vortex or shake to emulsify with the sedimentation cone pointing upwards.
Step 3: CENTRIFUGATION
Invert the Mini Parasep® SF and centrifuge at 400g for two minutes. Mini Parasep® SF fits all 15ml centrifuge buckets.
Step 4: EXAMINATION
Unscrew and discard the filter and mixing tube. Pour off all the liquid above the sediment. Pipette one drop of sediment onto a slide and cover with coverslip. Alternatively, follow laboratory SOP for slide preparation.