Step 1: SAMPLE PREPARATION
For empty Parasep®, unscrew lid and add 6.0ml of fixative and one drop of surfactant (eg: Apacor Triton X solution) to the mixing chamber. Alternatively use the reagent ready Midi Parasep®. Introduce a pea sized faecal sample to the fixative. Add 2.0ml of Ethyl Acetate to the mixing chamber. Mix in thoroughly with the Midi Parasep® spoon. If the sample is hard, break it up with the end of the spoon.
Step 2: EMULSIFICATION
Seal the Midi Parasep® by screwing in the filter/ sedimentation cone unit. Vortex or shake to emulsify with the sedimentation cone pointing upwards.
Step 3: CENTRIFUGATION
Invert the Midi Parasep® and centrifuge at 1200g for three minutes. Midi Parasep® fits all 50ml centrifuge buckets.
Step 4: EXAMINATION
Unscrew and discard the filter and mixing tube. Pour off all the liquid above the sediment. Pipette one drop of sediment onto a slide and cover with coverslip. Alternatively, follow laboratory SOP for slide preparation.