MIDI PARASEP®
Step 1: SAMPLE PREPARATION
A) Unscrew lid.
B) Add 6.0ml of fixative.
C) Add one drop of surfactant (eg:Triton X-100) to the chamber.
D) Introduce a level scoop of formed faecal sample (equivalent 1g)
to the fixative using the spoon at the end of the Midi Parasep®
filter. For liquid samples, add 2 level scoops (equivalent 2g) to
the fixative. Add 2.0ml of Ethyl Acetate to the mixing chamber.
Mix in thoroughly with the Midi Parasep® spoon. If the sample is
hard, break it up with the end of the spoon.
Step 2: EMULSIFICATION
A) Seal Midi Parasep® by screwing in the filter/sedimentation cone unit. Vortex or shake to emulsify with the sedimentation cone pointing upwards.
Step 3: CENTRIFUGATION
A) Invert Midi Parasep® and centrifuge at 1200g for 3 minutes. Midi Parasep® fits all 50ml centrifuge buckets.
Step 4: EXAMINATION
Open very slowly to avoid aerosol release.
A) Unscrew and discard the filter and mixing tube.
B) Pour off all the liquid above the sediment.
C) Pipette one drop of saline or Lugol’s Iodine solution onto a slide, add one drop of deposit to the saline or Lugol’s Iodine, mix sample and cover with cover-slip.