MINI PARASEP® SF PROTOCOL
Step 1: PREPARATION
A) Unscrew lid.
B) Add 3.3ml of fixative.
C) Add one drop of surfactant (eg: Apacor Triton X solution)
to the chamber.
Alternatively, use reagent ready Mini Parasep® SF
D) IIntroduce a level scoop of formed faecal sample (equivalent 0.5g) to the fixative using the spoon at the end of the Mini Parasep® SF filter. For liquid samples, add 2 level scoops (equivalent 1g) to the fixative.Mix in thoroughly with the Mini Parasep® SF spoon.
If the sample is hard, break it up with the end of the spoon.
Step 2: EMULSIFICATION
A) Seal Mini Parasep® SF by screwing in the filter/sedimentation cone
unit. Vortex or shake to emulsify with the sedimentation cone
pointing upwards.
Step 3: CENTRIFUGATION
A) Invert Mini Parasep® SF and centrifuge at 400g for 2 minutes. (J. Clin. Microbiol. doi:10.1128/JCM.00838-15)
Mini Parasep® SF fits all 15ml centrifuge buckets.
Step 4: EXAMINATION
A) Unscrew and discard the filter and mixing tube.
B) Pour off all the liquid above the sediment.
C) Pipette one drop of sediment onto a slide and cover with coverslip.
Alternatively, follow laboratory SOP for slide preparation.